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Collagen synthesis of human arterial smooth muscle cells: effects of platelet-derived growth factor, transforming growth factor-beta 1 and interleukin-1.

Abstract
The effects of platelet-derived growth factor (PDGF), transforming growth factor-beta 1 (TGF-beta 1) and interleukin-1 (IL-1) on collagen synthesis of cultured human arterial smooth muscle cells in a confluent state were investigated. Synthetic activity of collagenous protein was determined with [3H]-proline uptake, and subsequent analysis of collagen types by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by fluorography. Although PDGF (0.5 U/mL and 5.0 U/mL) enhanced total collagen synthesis per dish, it suppressed total collagen synthesis per DNA (DNA content in a dish). TGF-beta 1 (10 pmol/L and 100 pmol/L) enhanced total collagen synthesis both per dish and per DNA. IL-1 (0.1 U/mL and 1.0 U/mL) suppressed total collagen synthesis both per dish and per DNA. A fluorogram revealed that human arterial smooth muscle cells synthesize types I, III, IV and V collagen. Densitometric analysis showed PDGF suppressed the proportion of type IV collagen and increased that of type V collagen. TGF-beta 1 increased the proportions of types IV and V collagen. IL-1 elicited un- remarkable change in the proportion of collagen types. These results suggest that, in the event of human atherosclerosis, TGS-beta 1 is most effective in enhancing collagen synthesis, and PDGF modulates collagen metabolism by stimulating a cell division of smooth muscle cells with a resultant increase of collagenous protein, especially of type V collagen.
AuthorsY Okada, S Katsuda, H Watanabe, I Nakanishi
JournalActa pathologica japonica (Acta Pathol Jpn) Vol. 43 Issue 4 Pg. 160-7 (Apr 1993) ISSN: 0001-6632 [Print] Australia
PMID8493867 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Interleukin-1
  • Platelet-Derived Growth Factor
  • Transforming Growth Factor beta
  • Collagen
  • DNA
Topics
  • Adult
  • Aorta
  • Cells, Cultured
  • Collagen (biosynthesis, drug effects)
  • DNA (analysis)
  • Female
  • Humans
  • Interleukin-1 (pharmacology)
  • Male
  • Muscle, Smooth, Vascular (drug effects, metabolism)
  • Platelet-Derived Growth Factor (pharmacology)
  • Transforming Growth Factor beta (pharmacology)

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