Abstract | PURPOSE: To examine the effect of several agents that either stimulate or inhibit neovascularization on plasminogen activator inhibitor-1 and urokinase in retinal pigmented epithelial cells and vascular endothelial cells. METHODS: Steady-state levels of messenger RNA were assessed by Northern blots and dot blots and protein levels were assessed by immunoprecipitation. RESULTS: CONCLUSIONS: These data suggest that retinal pigmented epithelial cells may help to alter proteolytic activity in the subretinal space and thereby participate, along with endothelial cells, in the regulation of choroidal neovascularization.
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Authors | S F Hackett, P A Campochiaro |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 34
Issue 6
Pg. 2055-61
(May 1993)
ISSN: 0146-0404 [Print] United States |
PMID | 8491554
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Biological Factors
- Plasminogen Activator Inhibitor 1
- RNA, Messenger
- Dexamethasone
- Thrombin
- Urokinase-Type Plasminogen Activator
- Tetradecanoylphorbol Acetate
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Topics |
- Aged
- Biological Factors
(pharmacology)
- Blotting, Northern
- Cells, Cultured
- Dexamethasone
(pharmacology)
- Electrophoresis, Agar Gel
- Endothelium, Vascular
(drug effects, metabolism)
- Humans
- Infant
- Middle Aged
- Pigment Epithelium of Eye
(drug effects, metabolism)
- Plasminogen Activator Inhibitor 1
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Tetradecanoylphorbol Acetate
(pharmacology)
- Thrombin
(pharmacology)
- Urokinase-Type Plasminogen Activator
(genetics, metabolism)
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