Abstract |
We investigated NK cell infiltration into tumor developing lesions at early stage of tumor development after intraperitoneal inoculation of 3LL lung carcinoma into syngeneic C57BL/6 mice. Natural killer (NK) cells, which were detected by anti-NK 1.1 monoclonal antibody (mAb), remarkably increased in number in tumor-developing lesions (peritoneal cavity) as early as day 3 after inoculation of 3LL. The tumor-infiltrating NK cells from 3LL-inoculated mice produced a high level of interferon-gamma by co-culture with 3LL and showed enhanced cytotoxic activities against both NK-sensitive (YAC-1) and NK-resistant (3LL and P815) tumors. Furthermore, mice depleted of NK cells by injection of anti-NK 1.1 mAb or anti- asialo GM1 antibody showed shorter survival times after intraperitoneal inoculation of 3LL when compared with control mice. These results suggest that NK cells infiltrate the tumor-developing lesion at an early stage and may participate in the early protection against tumors through production of a high amount of interferon-gamma and enhanced cytotoxicity at tumor-bearing sites.
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Authors | S Kurosawa, G Matsuzaki, M Harada, T Ando, K Nomoto |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 23
Issue 5
Pg. 1029-33
(May 1993)
ISSN: 0014-2980 [Print] Germany |
PMID | 8477798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Surface
- Interferon-gamma
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Topics |
- Animals
- Antigens, Surface
(analysis)
- Cytotoxicity, Immunologic
- Female
- Interferon-gamma
(biosynthesis)
- Killer Cells, Natural
(immunology)
- Lung Neoplasms
(immunology)
- Lymphocyte Activation
- Lymphocytes, Tumor-Infiltrating
(immunology)
- Mice
- Mice, Inbred C57BL
- Mice, Inbred DBA
- Neoplasms, Experimental
(immunology, mortality)
- T-Lymphocytes, Cytotoxic
(immunology)
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