Abstract |
The gene encoding the envelope glycoprotein of a recent Danish isolate of a salmonid rhabdovirus, viral haemorrhagic septicaemia virus (VHSV) has been cloned and sequenced at the cDNA level. When compared with the deduced sequence of a French isolate of VHSV, it was noted that there were 13 amino acid substitutions in the Danish virus. Amino acid homologies with the glycoprotein of a North American salmonid rhabdovirus (infectious haematopoietic necrosis virus) indicate a high degree of structural similarity between the two fish rhabdovirus glycoproteins. Results from partial enzymatic deglycosylation of the viral protein indicate that all four NXT/S sites found in the sequence are N-glycosylated in the virus. The glycoprotein, without the N-terminal leader sequence and C-terminal hydrophobic anchor segment, was expressed in Escherichia coli as a factor Xa protease-cleavable fusion protein. The purified and renatured viral part of the recombinant protein was able to elicit VHSV-specific antibodies and neutralizing antibody activity in serum when injected into rainbow trout.
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Authors | N Lorenzen, N J Olesen, P E Jørgensen, M Etzerodt, T L Holtet, H C Thøgersen |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 74 ( Pt 4)
Pg. 623-30
(Apr 1993)
ISSN: 0022-1317 [Print] England |
PMID | 8468553
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycoproteins
- Oligodeoxyribonucleotides
- Recombinant Fusion Proteins
- Viral Proteins
- Viral Structural Proteins
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Escherichia coli
- Fish Diseases
(prevention & control)
- Fluorescent Antibody Technique
- Genes, Viral
- Glycoproteins
(genetics)
- Immunization
- Molecular Sequence Data
- Oligodeoxyribonucleotides
(chemistry)
- Recombinant Fusion Proteins
(immunology)
- Rhabdoviridae
(genetics)
- Trout
(immunology, microbiology)
- Viral Proteins
(genetics)
- Viral Structural Proteins
(genetics)
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