Abstract |
Survival after lipopolysaccharide challenge (LD80, 20 mg.kg-1, i.p.) was significantly enhanced by previous treatment with a microdose of LPS (50 micrograms.kg-1, i.v.). When NG-monomethyl-L-arginine, a specific inhibitor of the formation of nitric oxide from L-arginine, was given 30 minutes before microdose, survival was significantly reduced. When we monitored the serum Tumor Necrosis Factor (TNF) levels in both groups a significant reduction of TNF level after the microdose was observed in mice previously treated with L-NMMA. The ability of L- NNMA to reduce TNF release was dose dependent.
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Authors | A Rojas, J Padrón, L Caveda, M Palacios, S Moncada |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 191
Issue 2
Pg. 441-6
(Mar 15 1993)
ISSN: 0006-291X [Print] United States |
PMID | 8461002
(Publication Type: Journal Article)
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Chemical References |
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- omega-N-Methylarginine
- Nitric Oxide
- Arginine
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Topics |
- Animals
- Arginine
(analogs & derivatives, metabolism, pharmacology)
- Dose-Response Relationship, Drug
- Lipopolysaccharides
(administration & dosage, therapeutic use, toxicity)
- Male
- Mice
- Mice, Inbred BALB C
- Nitric Oxide
(metabolism)
- Sepsis
(etiology, metabolism, prevention & control)
- Tumor Necrosis Factor-alpha
(metabolism)
- omega-N-Methylarginine
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