Abstract |
An 80-kDa protein labeled with [3H] myristic acid in C6 glioma and N1E-115 neuroblastoma cells has been identified as the myristoylated alanine-rich C kinase substrate ( MARCKS protein) on the basis of its calmodulin-binding, acidic nature, heat stability, and immunochemical properties. When C6 cells preincubated with [3H] myristate were treated with 200 nM 4 beta-12-O-tetradecanoylphorbol 13-acetate (beta-TPA), labeled MARCKS was rapidly increased in the soluble digitonin fraction (maximal, fivefold at 10 min) with a concomitant decrease in the Triton X-100-soluble membrane fraction. However, phosphorylation of this protein was increased in the presence of beta-TPA to a similar extent in both fractions (maximal, fourfold at 30 min). In contrast, beta-TPA-stimulated phosphorylation of MARCKS in N1E-115 cells was confined to the membrane fraction only and no change in the distribution of the myristoylated protein was noted relative to alpha-TPA controls. These results indicate that although phosphorylation of MARCKS by protein kinase C occurs in both cell lines, it is not directly associated with translocation from membrane to cytosol, which occurs in C6 cells only. The cell-specific translocation of MARCKS appears to correlate with previously demonstrated differential effects of phorbol esters on stimulation of phosphatidylcholine turnover in these two cell lines.
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Authors | D M Byers, F B Palmer, M W Spence, H W Cook |
Journal | Journal of neurochemistry
(J Neurochem)
Vol. 60
Issue 4
Pg. 1414-21
(Apr 1993)
ISSN: 0022-3042 [Print] England |
PMID | 8455032
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Calmodulin
- Intracellular Signaling Peptides and Proteins
- Marcks protein, mouse
- Marcks protein, rat
- Membrane Proteins
- Myristic Acids
- Proteins
- Myristic Acid
- Myristoylated Alanine-Rich C Kinase Substrate
- Protein Kinase C
- Tetradecanoylphorbol Acetate
- Calcium
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Topics |
- Animals
- Biological Transport
- Calcium
(pharmacology)
- Calmodulin
(metabolism)
- Cell Membrane
(metabolism)
- Cytosol
(metabolism)
- Drug Stability
- Glioma
(metabolism)
- Hot Temperature
- Hydrogen-Ion Concentration
- Intracellular Signaling Peptides and Proteins
- Kinetics
- Membrane Proteins
- Mice
- Myristic Acid
- Myristic Acids
(metabolism)
- Myristoylated Alanine-Rich C Kinase Substrate
- Neuroblastoma
(metabolism)
- Phosphorylation
- Protein Kinase C
(metabolism)
- Proteins
(metabolism)
- Rats
- Tetradecanoylphorbol Acetate
(pharmacology)
- Tumor Cells, Cultured
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