Cloning and sequence analysis of the structural pilin gene of Brazilian purpuric fever-associated Haemophilus influenzae biogroup aegyptius.

Abstract

We have cloned and sequenced the Brazilian purpuric fever (BPF)-associated Haemophilus influenzae biogroup aegyptius (Hae) pilin gene. The sequence contained a 648-bp open reading frame encoding a mature pilin protein of 191 amino acids with a calculated mass of 20.5 kDa. There was 82% homology between the open reading frames of the BPF strain F3031 and H. influenzae type b (Hib) (strain M43) pilin genes and 71% homology at the amino acid level between the mature pilin proteins. However, areas of diversity were noted throughout the gene. A 17-bp probe corresponding to an area of diversity in the N-terminal region of the BPF-associated gene hybridized with other BPF strains but not with non-BPF Hae or Hib. In summary, the pilin protein of BPF-associated Hae is highly homologous to Hib pilin yet remains structurally distinct.

• Authors: A M Whitney, M M Farley
• Journal: Infection and immunity (Infect Immun) Vol. 61 Issue 4 Pg. 1559-62 (Apr 1993) ISSN: 0019-9567 [Print] United States
• PMID: 8454364 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bacterial Outer Membrane Proteins
• Oligodeoxyribonucleotides
• Recombinant Proteins
• Fimbriae Proteins

Topics

• Amino Acid Sequence
• Bacterial Outer Membrane Proteins (genetics)
• Base Sequence
• Cloning, Molecular
• Fimbriae Proteins
• Genes, Bacterial
• Haemophilus Infections (microbiology)
• Haemophilus influenzae (genetics)
• Humans
• Molecular Sequence Data
• Oligodeoxyribonucleotides (chemistry)
• Purpura
• Recombinant Proteins
• Sequence Alignment