Mitomycin-induced synthesis of a Shiga-like toxin from enteropathogenic Escherichia coli H.I.8.

Abstract

Escherichia coli H.I.8, an O128 infant diarrhea isolate, produces low titers of a unique Shiga-like toxin (SLT), called SLT-IIva, which is a variant of SLT-II. We investigated induction of toxin synthesis and the putative association of a bacteriophage with toxin synthesis. Induction of broth cultures of strain H.I.8 with mitomycin yielded a 3,000-fold increase in SLT-IIva, production of a colicin, and appearance of a bacteriophage. Southern hybridization demonstrated that the genes for SLT-IIva were not carried by the bacteriophage.

• Authors: A J Yee, S De Grandis, C L Gyles
• Journal: Infection and immunity (Infect Immun) Vol. 61 Issue 10 Pg. 4510-3 (Oct 1993) ISSN: 0019-9567 [Print] United States
• PMID: 8406845 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• Oligodeoxyribonucleotides
• Shiga Toxin 1
• Shiga Toxin 2
• Mitomycin

Topics

• Bacterial Toxins (biosynthesis)
• Base Sequence
• Chromosomes, Bacterial (ultrastructure)
• Escherichia coli (genetics, pathogenicity)
• Gene Expression Regulation, Bacterial (drug effects)
• Genes, Bacterial
• Mitomycin (pharmacology)
• Molecular Sequence Data
• Oligodeoxyribonucleotides (chemistry)
• Shiga Toxin 1
• Shiga Toxin 2