Changes in creatine kinase M localization in acute ischemic myocardial cells. Immunoelectron microscopic studies.

Abstract

To clarify the changes in creatine kinase M localization along with the progress of myocardial ischemia, immunoelectron microscopic studies were performed using rabbit anti-canine creatine kinase M Fab'-horseradish peroxidase conjugate in 21 dogs. Myocardial ischemia was induced by occlusion of the left anterior descending coronary artery for 15 (n = 5), 30 (n = 5), 60 (n = 5), or 180 (n = 4) minutes. Two dogs were used as normal controls. As we have already demonstrated, most creatine kinase M in normal myocardial cells was localized over the entire A band in association with the thick filament, suggesting that creatine kinase in this region (A-band creatine kinase) was the enzyme coupled with myosin ATPase. After 15 minutes of ischemia, creatine kinase M showed only minimal changes in its location, indicating that A-band creatine kinase still has the ability to couple with myosin ATPase (reversible injury). However, after 30 minutes of ischemia, A-band creatine kinase diffused markedly to the I band (transitional phase), and after 60 minutes of ischemia, it leaked out to extracellular spaces (irreversible injury). After 180 minutes of ischemia, most A-band creatine kinase disappeared from the myocardial cells (coagulation necrosis). These features of creatine kinase M localization seemed to reflect each stage of ischemic cell injury. We conclude that myocardial ischemia results in a dissociation of creatine kinase molecules from the thick filament, which leads the energy transport system to destruction.

• Authors: N Otsu, I Yamaguchi, E Komatsu, K Miyazawa
• Journal: Circulation research (Circ Res) Vol. 73 Issue 5 Pg. 935-42 (Nov 1993) ISSN: 0009-7330 [Print] United States
• PMID: 8403263 (Publication Type: Journal Article)

Chemical References

• Isoenzymes
• Creatine Kinase
• Myosins

Topics

• Acute Disease
• Animals
• Creatine Kinase (analysis)
• Dogs
• Isoenzymes
• Microscopy, Immunoelectron
• Myocardial Ischemia (enzymology, pathology)
• Myocardium (enzymology, ultrastructure)
• Myosins (analysis)