Galactosyl hydroxylysine and deoxypyridinoline: a methodological comparison.

Abstract

Galactosyl hydroxylysine and deoxypyridinoline are at present the most promising markers of bone resorption. Various studies have indeed shown that these two markers discriminate with high accuracy subjects with different rates of bone turnover and that their accuracies and discriminate power are very similar. The aim of this paper is to compare the practicality and the reproducibility of the HPLC galactosyl hydroxylysine and deoxypyridinoline assays. In summary, this review shows that the galactosyl hydroxylysine and deoxypyridinoline HPLC assays differ mainly in the need, in using deoxypyridinoline, for an acid hydrolysis and a preextraction of the urine samples. This implies two major problems for deoxypyridinoline: 1) more time is required due to the cumbersome preanalytical procedures; and 2) a lower reproducibility. Our data, in fact, show that both the intra-assay and inter-assay coefficient of variation of the deoxypyridinoline assay are almost 100% higher than those of the galactosyl hydroxylysine assay.

• Authors: P Bettica, D J Baylink, L Moro
• Journal: European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies (Eur J Clin Chem Clin Biochem) Vol. 31 Issue 7 Pg. 459-65 (Jul 1993) ISSN: 0939-4974 [Print] Germany
• PMID: 8399787 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Biomarkers
• Hydroxylysine
• galactosylhydroxylysine
• deoxypyridinoline

Topics

• Adult
• Amino Acids (urine)
• Biomarkers (urine)
• Bone Resorption (urine)
• Child
• Chromatography, High Pressure Liquid
• Female
• Humans
• Hydroxylysine (analogs & derivatives, urine)
• Molecular Weight
• Reproducibility of Results