Interaction between T cells and murine acquired immunodeficiency virus superantigen: effect of second signal on T cell reactivity to the MAIDS virus superantigen.

Abstract

A murine acquired immunodeficiency (MAIDS) virus transformed B cell line, B6-1710, was shown to express superantigen activity and stimulate T cell hybridomas to produce IL-2. However, T cell clones and lines from which B6-1710 reactive hybridomas were established failed to proliferate upon stimulation with B6-1710, while B6-1710 cells were capable of presenting bacterial superantigen to stimulate both T cell hybridomas and clones. Proliferative response of T cells to MAIDS virus superantigen could be detected by the addition of the pharmacological agent phorbol myristate acetate (PMA). Thus, B6-1710 seems to lack a stimulatory activity necessary for the proliferative response of T cells to the MAIDS viral superantigen. In the analysis of the response of naive splenic T cells to the MAIDS superantigen, T cells recovered from a culture stimulated with B6-1710 cells in the presence of PMA showed no dominant TCR V beta chain usage, while cells recovered from a culture stimulated with B6-1710 alone were dominated by T cells expressing V beta 5, 11, and 12 TCR chains. These results suggest that T cells bearing a majority of TCR V beta chains are capable of responding to B6-1710 superantigen. The avidity of interaction between T cells and viral superantigen may differ significantly among T cells and those T cells exhibiting weak interaction with MAIDS virus superantigen may require additional signals, such as PMA, for an in vitro proliferative response to B6-1710 cells.

• Authors: M Heise, K Chow, O Kanagawa
• Journal: International immunology (Int Immunol) Vol. 5 Issue 6 Pg. 583-90 (Jun 1993) ISSN: 0953-8178 [Print] England
• PMID: 8394108 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral
• Receptors, Antigen, T-Cell, alpha-beta
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Antigen-Presenting Cells (immunology)
• Antigens, Viral
• B-Lymphocytes (immunology)
• Cell Line, Transformed
• Hybridomas (immunology)
• Leukemia Virus, Murine (immunology)
• Lymphocyte Activation (drug effects)
• Mice
• Mice, Inbred C57BL
• Mice, Inbred CBA
• Receptors, Antigen, T-Cell, alpha-beta
• Signal Transduction (immunology)
• T-Lymphocytes (drug effects, immunology)
• Tetradecanoylphorbol Acetate (pharmacology)