Mechanism of protective immunity induced by porin-lipopolysaccharide against murine salmonellosis.

Abstract

Investigations were undertaken to characterize the protective immunity induced by porin-lipopolysaccharide (LPS) against Salmonella typhimurium infection in mice. Mice immunized with porin-LPS showed higher levels of antiporin immunoglobulin G than mice which received porin alone. Further, T cells from porin-LPS-immunized mice showed an augmented proliferative response to porin in vitro compared with the response of T cells from porin-injected animals. The passive transfer of anti-LPS antibodies conferred significant protection (17%), while antiporin serum failed to protect mice against lethal challenge, indicating the protective ability of anti-LPS antibodies. However, the transfer of serum obtained from porin-LPS-immunized mice resulted in better protection (30%) than did anti-LPS or antiporin antibodies alone. In contrast to LPS, monophosphoryl lipid A completely failed to induce protection against lethal infection. However, comparable to the effect of LPS, injection of porin with monophosphoryl lipid A enhanced antibody response and the protective ability of porin (81.25%). The transfer of T cells from porin-LPS-immunized mice provided higher levels of protection (47%) against lethal challenge than did T cells from porin-immunized mice (23%). The combination of T cells and serum from porin-immunized mice transferred 36% protection. However, a combination of T cells and serum from porin-LPS-immunized mice conferred the highest level of protection (92%), which was reflected by the number of survivors (100%) in the porin-LPS-immunized group. These results demonstrate that besides the protective effect of anti-LPS antibodies, the ability of LPS to augment humoral and cell-mediated immune responses to porin confers effective protection against Salmonella infection.

• Authors: S Muthukkumar, V R Muthukkaruppan
• Journal: Infection and immunity (Infect Immun) Vol. 61 Issue 7 Pg. 3017-25 (Jul 1993) ISSN: 0019-9567 [Print] United States
• PMID: 8390406 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Bacterial Outer Membrane Proteins
• Immune Sera
• Immunoglobulin G
• Immunoglobulin M
• Lipid A
• Lipopolysaccharides
• Porins
• monophosphoryl lipid A

Topics

• Animals
• Antibodies, Bacterial (blood)
• Bacterial Outer Membrane Proteins (immunology)
• Female
• Immune Sera (immunology)
• Immunization
• Immunization, Passive
• Immunoglobulin G (blood)
• Immunoglobulin M (blood)
• Lipid A (analogs & derivatives, pharmacology)
• Lipopolysaccharides (immunology, pharmacology)
• Lymphocyte Activation
• Male
• Mice
• Mice, Inbred BALB C
• Porins
• Salmonella Infections, Animal (immunology)
• T-Lymphocytes (immunology)