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Implication of superoxide radicals on ischemia-reperfusion-induced skeletal muscle injury in rats.

Abstract
This study was designed to clarify the mechanism of ischemia-reperfusion injury to skeletal muscle using long-acting polyoxyethylene-modified superoxide dismutase (SOD-POE) in rats. The gastrocnemius muscles of male Lews rats were investigated. Tissue levels of ATP decreased to 20% of nonischemic values after 3 h ischemia and returned to 94% after 1 h reperfusion. In contrast, they decreased to 3.5% after 4 h ischemia and remained at 20% after 1 h reperfusion. Although SOD-POE did not affect the decrease of ATP during ischemia, it improved significantly the recovery of ATP: 28%. Tissue levels of lipid peroxides (LPO) after 3 h ischemia and 1 h reperfusion did not change significantly compared with the nonischemic levels (0.71 +/- 0.32 nmol/mg protein, mean +/- SD). They showed no increase after 4 h ischemia, but increased explosively after 1 h reperfusion (2.15 +/- 0.73 nmol/mg protein). SOD-POE did not affect LPO levels during ischemia but prevented the increase of LPO significantly after reperfusion (0.98 +/- 0.25 nmol/mg protein). Xanthine oxidase activity did not increase after 3 h ischemia (22.3 +/- 7.0 mU/g) compared with the nonischemic values (17.6 +/- 10.0 mU/g). In contrast, it increased 2.5-fold after 4 h ischemia (50.1 +/- 13.7 mU/g) and remained at a significantly high level after 1 h reperfusion. SOD-POE did not affect xanthine oxidase activity during ischemia and reperfusion. These results suggest that lipid peroxidation by superoxide radicals produced by xanthine oxidase is a contributory factor to ischemia-reperfusion injury to skeletal muscle, and the clinical application of SOD-POE might be expected.
AuthorsS Kawasaki, S Sugiyama, N Ishiguro, T Ozawa, T Miura
JournalEuropean surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes (Eur Surg Res) 1993 May-Jun Vol. 25 Issue 3 Pg. 129-36 ISSN: 0014-312X [Print] Switzerland
PMID8388794 (Publication Type: Journal Article)
Chemical References
  • Adenine Nucleotides
  • Free Radical Scavengers
  • Free Radicals
  • Lipid Peroxides
  • Phosphocreatine
  • Superoxides
  • Polyethylene Glycols
  • Superoxide Dismutase
  • polyethylene glycol-superoxide dismutase
  • Xanthine Oxidase
Topics
  • Adenine Nucleotides (metabolism)
  • Animals
  • Free Radical Scavengers
  • Free Radicals
  • Ischemia (metabolism)
  • Lipid Peroxides (metabolism)
  • Male
  • Muscles (blood supply, injuries, metabolism)
  • Phosphocreatine (metabolism)
  • Polyethylene Glycols (pharmacology)
  • Rats
  • Rats, Inbred Lew
  • Reperfusion Injury (etiology, metabolism, prevention & control)
  • Superoxide Dismutase (pharmacology)
  • Superoxides (metabolism)
  • Xanthine Oxidase (metabolism)

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