Transcription factor TFIID recognizes DNA sequences downstream of the TATA element in the Hsp70 heat shock gene.

Abstract

The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters.

• Authors: P A Emanuel, D S Gilmour
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 90 Issue 18 Pg. 8449-53 (Sep 15 1993) ISSN: 0027-8424 [Print] United States
• PMID: 8378317 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA-Binding Proteins
• Heat-Shock Proteins
• Recombinant Proteins
• TATA-Box Binding Protein
• Transcription Factor TFIID
• Transcription Factors
• DNA

Topics

• Animals
• Base Sequence
• DNA (genetics, isolation & purification, metabolism)
• DNA-Binding Proteins (isolation & purification, metabolism)
• Drosophila (genetics, metabolism)
• Electrophoresis, Polyacrylamide Gel
• Heat-Shock Proteins (genetics)
• Promoter Regions, Genetic
• Recombinant Proteins (metabolism)
• Restriction Mapping
• Sequence Deletion
• Substrate Specificity
• TATA Box
• TATA-Box Binding Protein
• Transcription Factor TFIID
• Transcription Factors (isolation & purification, metabolism)