This study was focused on the changes of high energy
phosphates and tissue blood flow (TBF) in
ischemia and reperfusion, of jaundiced rat liver. We ligated and cut the bile duct of male Wistar rats to induce
jaundice. The rats were divided into two groups, BDL-4W which had
ligation four weeks before examination and control which had no
ligation. Two studies were performed; In study A,
ischemia was induced by clamping portal vein and hepatic artery for 60 minutes. In study B, 15 minutes of
ischemia was repeated four times with intervals of 10 minutes of reperfusion. A: 1) In BDL-4W,
ATP was significantly lower than that in control at pre-
ischemia and reperfusion. Recovery ratios (RR) of
ATP in BDL-4W were significantly lower than those in control. 2) RR or TBF in BDL-4W were significantly lower than those in control. 3) In BDL-4W,
xanthine was significantly higher than that in control at reperfusion. B: 4) There were no significant differences in the RR of
ATP after reperfusion between two groups. 5) There were no significant differences in the RR of TBF between two groups. 6) There were no significant differences in
xanthine after reperfusion between two groups. We concluded that intermittent brief
ischemia was useful when 60 minutes of
ischemia was required for the operation of jaundiced liver.