Abstract |
A novel monoclonal antibody, YB-2 was obtained after immunization of mice with fucosylated antigens isolated from human saliva. The antibody was demonstrated to react with Y (Fuc alpha 1-->2Gal-beta 1-->4[Fuc alpha 1-->3]GlcNAc beta),Leb (Fuc alpha 1-->2Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta) and H type 2 (Fuc alpha 1-->2Gal-beta 1-->4GlcNAc beta) antigens, but not with H type 1 (Fuc alpha 1-->2Gal beta 1-->3GlcNAc beta), Lea (Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta), X (Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc beta) or with non-fucosylated antigens. Inhibition assays of YB-2 antibody with such reactive antigens showed that YB-2 antibody preferentially reacted with Y antigen. Formalin-fixed and paraffin-embedded sections prepared from normal and malignant colorectal tissues were examined immunohistochemically with YB-2. The positive rates of staining with YB-2 antibody were 88.6% in malignant and 12.0% in normal tissues. The expression of fucosylated antigens detected by YB-2 antibody seemed to be correlated with survival among patients with primary colorectal cancer. Therefore, YB-2 antibody could be useful as an immunochemical tool for diagnosis and evaluation of the prognosis of colorectal cancer.
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Authors | S Yazawa, S Akamatsu, T Tachikawa, H Naito, J Nakamura, T Asao, Y Nagamachi, T Nakajima, S Shin, D Chia |
Journal | Japanese journal of cancer research : Gann
(Jpn J Cancer Res)
Vol. 84
Issue 6
Pg. 641-8
(Jun 1993)
ISSN: 0910-5050 [Print] Japan |
PMID | 8340252
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Neoplasm
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Topics |
- Antibodies, Monoclonal
(isolation & purification)
- Antibody Specificity
- Antigens, Neoplasm
(analysis)
- Colorectal Neoplasms
(diagnosis, immunology, mortality)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunoenzyme Techniques
- Survival Analysis
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