Abstract |
The control of bovine trypanosomiasis in Africa continues to rely heavily on the chemoprophylactic drug isometamidium ( ISMM) chloride. However, despite many years of use, no methods are available that are sufficiently sensitive to measure drug levels in treated cattle. An enzyme-linked immunosorbent assay (ELISA) for the detection of ISMM in the serum of treated cattle has been developed and evaluated. Liquid-phase ISMM (sample) competes with solid-phase bound ISMM- protein conjugate for biotinylated sheep anti- ISMM IgG. The specific IgG is detected by streptavidin- peroxidase, using tetramethylbenzidine for colour development. Assay calibration is by four-parameter logistic curve-fitting. Factors contributing to absorbance variance were considered in assay optimization and improvement of precision and the lower limit of detection (approximately 0.1 ng/ml in serum). The ELISA was shown to detect serum ISMM for several months after treatment of cattle in a trypanosomiasis endemic country. The potential uses of this assay include the development of rational prophylactic drug regimens, and the indirect detection of drug-resistant trypanosomes.
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Authors | M C Eisler, E A Gault, H V Smith, A S Peregrine, P H Holmes |
Journal | Therapeutic drug monitoring
(Ther Drug Monit)
Vol. 15
Issue 3
Pg. 236-42
(Jun 1993)
ISSN: 0163-4356 [Print] United States |
PMID | 8333004
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Bacterial Proteins
- Benzidines
- Phenanthridines
- Trypanocidal Agents
- 3,3',5,5'-tetramethylbenzidine
- Biotin
- isometamidium chloride
- Streptavidin
- Peroxidases
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Topics |
- Animals
- Antibodies
- Antibody Specificity
- Bacterial Proteins
- Benzidines
- Biotin
- Calibration
- Cattle
- Enzyme-Linked Immunosorbent Assay
- Evaluation Studies as Topic
- Female
- Male
- Peroxidases
- Phenanthridines
(blood)
- Sheep
- Streptavidin
- Trypanocidal Agents
(blood)
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