Expression of cytochrome b558 on B cell- and CD 30 positive-lymphomas.

Abstract

Expression of cytochrome b558, an essential constituent of the superoxide generating system in phagocytes, was demonstrated in B-lymphocytes. To determine its expression in malignant lymphoma (ML), 103 non-Hodgkin's MLs and 18 Hodgkin's (HD) MLs, together with non-tumorous lymphoid tissues were immunohistochemically analyzed, using two antibodies specific for the cytochrome. In non-tumorous tonsils and lymph nodes, B-lymphocytes, especially in the mantle zones, and histiocytes stained heavily, while T-lymphocytes failed to stain. Among the 55 B-MLs, all follicular lymphomas (9/9) and 18/46 of the diffuse lymphomas were found to express cytochrome b558. Among 48 T-MLs, 46 were unstained by antibodies against the cytochrome. The two cytochrome b558-positive cases were CD 30-positive anaplastic large cell lymphomas. Sixteen of 18 HD patients had CD 30-positive Reed-Sternberg cells which also stained with antibody alpha LC (an anticytochrome b558 antibody). Thus, cytochrome b558, represents a new lymphocyte differentiation antigen.

• Authors: M Shiota, S Mori, S Imajoh-Ohmi, M Nakamura, S Kanegasaki, H Serizawa, T Izumo, T Uehara
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 189 Issue 9 Pg. 985-91 (Nov 1993) ISSN: 0344-0338 [Print] Germany
• PMID: 8302731 (Publication Type: Journal Article)

Chemical References

• Cytochrome b Group
• Ki-1 Antigen
• cytochrome b558
• NADPH Oxidases

Topics

• B-Lymphocytes (chemistry)
• Cytochrome b Group (analysis)
• Hodgkin Disease (metabolism, pathology)
• Humans
• Immunohistochemistry
• Immunophenotyping
• Ki-1 Antigen (analysis)
• Lymphoid Tissue (chemistry)
• Lymphoma, B-Cell (chemistry, pathology)
• Lymphoma, Non-Hodgkin (chemistry, pathology)
• Lymphoma, T-Cell (chemistry, pathology)
• NADPH Oxidases
• Reed-Sternberg Cells (chemistry)
• T-Lymphocytes (chemistry)