Abstract |
The novelty of applying three-dimensional graphic capabilities involving area and vector changes was used to understand variations in inositol derivatives and their comodulating influence on calcium (Ca2+) in skeletal muscle under the duress of burn trauma. Burn injury was achieved by scalding of predefined areas (0, 20% and 50%) on the dorsal and ventral surfaces of mice. At day 21, 45Ca2+ influx and efflux procedures were performed. Through multiple regression, the dependency of intracellular Ca2+ was determined with respect to three polyinositol forms each representing independence simultaneously. The contribution of each of these parameters was assigned to a three-dimensional axis. Vector analysis determined the relationship of intracellular Ca2+ to each of the independent parameters in control, 20% and 50% burn groups. Such vector analysis allows for a clear visualization of the interrelationships that exist between secondary (viz, IP3) and tertiary (viz, Ca2+) messenger systems. This clear visualization may allow for a greater understanding of messenger systems that may lead to more effective treatment of the systemic effects of severe burn trauma.
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Authors | J F Tomera, K Lilford |
Journal | Methods and findings in experimental and clinical pharmacology
(Methods Find Exp Clin Pharmacol)
Vol. 15
Issue 7
Pg. 471-82
(Sep 1993)
ISSN: 0379-0355 [Print] Spain |
PMID | 8255127
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Inositol Phosphates
- Calcium
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Topics |
- Animals
- Burns
(metabolism, physiopathology)
- Calcium
(metabolism)
- Inositol Phosphates
(metabolism)
- Male
- Mice
- Mice, Inbred Strains
- Models, Biological
- Multivariate Analysis
- Muscles
(metabolism, physiopathology)
- Regression Analysis
- Signal Transduction
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