Cloning and sequencing of two new Verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea.

Abstract

We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the VT2, VT2vha, VT2vhb, SLT-IIv (VT2vp1) and SLT-IIva (VT2vp2) genes. Their nucleotide sequences were much more closely homologous to that of VT2vh than to that of VT2vp. Search for these two new genes in other Verocytotoxin-producing E. coli strains resulted in the isolation of 2 strains carrying one of the new VT2 variant genes, one strain from Tokyo and the other from Canada.

• Authors: Z Lin, S Yamasaki, H Kurazono, M Ohmura, T Karasawa, T Inoue, S Sakamoto, T Suganami, T Takeoka, Y Taniguchi
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 37 Issue 6 Pg. 451-9 ( 1993) ISSN: 0385-5600 [Print] Australia
• PMID: 8231960 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• DNA Probes
• DNA, Bacterial
• Enterotoxins
• Shiga Toxin 2

Topics

• Animals
• Bacterial Toxins (genetics)
• Base Sequence
• Cattle
• Cattle Diseases (microbiology)
• Cloning, Molecular
• DNA Probes
• DNA, Bacterial (genetics)
• Diarrhea (microbiology, veterinary)
• Enterotoxins (genetics)
• Escherichia coli (genetics)
• Escherichia coli Infections (microbiology)
• Genetic Variation
• Humans
• Molecular Sequence Data
• Restriction Mapping
• Sequence Homology
• Shiga Toxin 2
• Vero Cells