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Altered hepatic production of apolipoproteins B and E in the fasted septic rat: factors in the development of hypertriglyceridemia.

Abstract
The etiology of hypertriglyceridemia associated with sepsis remains unclear, but we will attempt to elucidate its character by studying the hepatic production of apolipoproteins B and E. Male Lewis rats (260-330 g) were assigned to two groups, control (n = 5) and septic (n = 5). The septic group was injected with 2 x 10(8) live Escherichia coli colonies/100 g body wt. Food was removed from all rats after injections. Twenty-four hours later a recirculating in situ liver perfusion was performed for 120 min with KRB buffer, containing L-[35S]methionine. The production of apolipoprotein B (apo B), apolipoprotein E (apo E), albumin, and transferrin was determined by immunoprecipitation. The septic rats showed a protein-specific response to sepsis. The total protein secreted increased throughout each perfusion, septic greater than control. Apo B production was increased 2.6-fold in the septic versus control groups (P = 0.037), while apo E production was decreased by 2.9 times control (P = 0.036). Albumin production was decreased 2-fold in the septic group (P = 0.002). The increased hepatic production of apo B represents an increased number of very low density lipoprotein (VLDL) particles and contributes to the elevated VLDL triglyceride levels seen in sepsis. In contrast, decreased apo E production may result in a diminished ability for peripheral and/or hepatic receptor recognition of VLDL and VLDL remnants, respectively. Each of these changes are factors in the development of hypertriglyceridemia in sepsis.
AuthorsR J Tripp, A Tabares, H Wang, S Lanza-Jacoby
JournalThe Journal of surgical research (J Surg Res) Vol. 55 Issue 5 Pg. 465-72 (Nov 1993) ISSN: 0022-4804 [Print] United States
PMID8231164 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Albumins
  • Apolipoproteins B
  • Apolipoproteins E
  • Lipoproteins, VLDL
  • Transferrin
  • Triglycerides
  • very low density lipoprotein triglyceride
Topics
  • Albumins (biosynthesis)
  • Animals
  • Apolipoproteins B (biosynthesis)
  • Apolipoproteins E (biosynthesis)
  • Bacteremia (complications, metabolism)
  • Escherichia coli Infections (complications, metabolism)
  • Fasting
  • Hypertriglyceridemia (etiology)
  • Immunosorbent Techniques
  • Lipoproteins, VLDL (blood)
  • Liver (metabolism)
  • Male
  • Perfusion
  • Protein Biosynthesis
  • Rats
  • Rats, Inbred Lew
  • Transferrin (biosynthesis)
  • Triglycerides (blood)

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