Abstract |
The etiology of hypertriglyceridemia associated with sepsis remains unclear, but we will attempt to elucidate its character by studying the hepatic production of apolipoproteins B and E. Male Lewis rats (260-330 g) were assigned to two groups, control (n = 5) and septic (n = 5). The septic group was injected with 2 x 10(8) live Escherichia coli colonies/100 g body wt. Food was removed from all rats after injections. Twenty-four hours later a recirculating in situ liver perfusion was performed for 120 min with KRB buffer, containing L-[35S] methionine. The production of apolipoprotein B ( apo B), apolipoprotein E ( apo E), albumin, and transferrin was determined by immunoprecipitation. The septic rats showed a protein-specific response to sepsis. The total protein secreted increased throughout each perfusion, septic greater than control. Apo B production was increased 2.6-fold in the septic versus control groups (P = 0.037), while apo E production was decreased by 2.9 times control (P = 0.036). Albumin production was decreased 2-fold in the septic group (P = 0.002). The increased hepatic production of apo B represents an increased number of very low density lipoprotein (VLDL) particles and contributes to the elevated VLDL triglyceride levels seen in sepsis. In contrast, decreased apo E production may result in a diminished ability for peripheral and/or hepatic receptor recognition of VLDL and VLDL remnants, respectively. Each of these changes are factors in the development of hypertriglyceridemia in sepsis.
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Authors | R J Tripp, A Tabares, H Wang, S Lanza-Jacoby |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 55
Issue 5
Pg. 465-72
(Nov 1993)
ISSN: 0022-4804 [Print] United States |
PMID | 8231164
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Albumins
- Apolipoproteins B
- Apolipoproteins E
- Lipoproteins, VLDL
- Transferrin
- Triglycerides
- very low density lipoprotein triglyceride
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Topics |
- Albumins
(biosynthesis)
- Animals
- Apolipoproteins B
(biosynthesis)
- Apolipoproteins E
(biosynthesis)
- Bacteremia
(complications, metabolism)
- Escherichia coli Infections
(complications, metabolism)
- Fasting
- Hypertriglyceridemia
(etiology)
- Immunosorbent Techniques
- Lipoproteins, VLDL
(blood)
- Liver
(metabolism)
- Male
- Perfusion
- Protein Biosynthesis
- Rats
- Rats, Inbred Lew
- Transferrin
(biosynthesis)
- Triglycerides
(blood)
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