Abstract |
When OK-432, a well-known streptococcal preparation for an anti-tumour drug, was administered into the pleural cavity of patients with malignant pleurisy, a rapid and prominent leukocytosis, predominantly consisting of neutrophils, was observed in the cavity. Neutrophil infiltration usually peaked 6-9 h after OK-432 administration, and levelled down after 24 h. Prior to the neutrophil accumulation, transient but marked elevation of various inflammatory cytokine levels including IL-1 beta, TNF-alpha, IL-8 and G-CSF was observed. In particular, IL-8 levels increased more than 10-fold, while GM-CSF did not change significantly. A good correlation between IL-8 levels and neutrophil chemotactic response was observed particularly during 0-3 h. Specific neutralization or removal of IL-8 by antibody column abrogated half of the neutrophil chemotaxis, while neutralization of C5a removed around 40%. Sequential removal of IL-8 and C5a abrogated totally 80% of chemotaxis, confirming that these two factors are mostly responsible for the neutrophil chemotaxis in the pleural fluids. These results have suggested that rapid neutrophil infiltration induced by OK-432 in vivo is ascribable largely to IL-8 and in part to C5a.
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Authors | I Tsuchiya, T Kasahara, K Yamashita, Y C Ko, K Kanazawa, K Matsushima, N Mukaida |
Journal | Cytokine
(Cytokine)
Vol. 5
Issue 6
Pg. 595-603
(Nov 1993)
ISSN: 1043-4666 [Print] England |
PMID | 8186372
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemotactic Factors
- Cytokines
- Interleukin-1
- Interleukin-8
- Tumor Necrosis Factor-alpha
- Picibanil
- Complement C5a
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Topics |
- Adult
- Aged
- Chemotactic Factors
(biosynthesis, isolation & purification)
- Chemotaxis, Leukocyte
(immunology)
- Chromatography, Agarose
- Complement C5a
(metabolism)
- Cytokines
(biosynthesis)
- Humans
- Interleukin-1
(metabolism)
- Interleukin-8
(metabolism)
- Male
- Middle Aged
- Neutralization Tests
- Neutrophils
(immunology, pathology)
- Picibanil
(administration & dosage)
- Pleural Effusion, Malignant
(immunology, pathology)
- Tumor Necrosis Factor-alpha
(metabolism)
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