Expression of NAD glycohydrolase activity by rat mammary adenocarcinoma cells transformed with rat T cell alloantigen RT6.2.

Abstract

RT6.2 is a 26-kDa alloantigen expressed only on post-thymic T cells and attached to the cell membrane through a glycosylphosphatidylinositol (GPI) anchor. It has been reported that expression of RT6.2 in animal models may correlate with lymphopenia and genetically-induced insulin-dependent diabetes mellitus. Its physiological function is unclear. Since RT6.2 has significant amino acid identity with a GPI-anchored rabbit muscle NAD:arginine ADP-ribosyltransferase, RT6.2 was expressed in rat mammary adenocarcinoma cells and the ability of the expressed protein to catalyze ADP-ribose transfer reactions was examined. Cells transformed with the RT6.2 gene expressed NAD glycohydrolase activity that was released from intact cells by phosphatidylinositol-specific phospholipase C, consistent with its presence on the cell surface. A similar activity was not detected with vector-transformed cells. RT6.2 did not ADP-ribosylate simple guanidino compounds. The molecular weight of the phosphatidylinositol-specific phospholipase C-released NAD glycohydrolase, determined by SDS-polyacrylamide gel electrophoresis, was 22,000-24,000, in good agreement with that of native RT6.2. These results strongly suggest that the rat T cell alloantigen RT6.2 is a GPI-anchored NAD glycohydrolase.

• Authors: T Takada, K Iida, J Moss
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 269 Issue 13 Pg. 9420-3 (Apr 01 1994) ISSN: 0021-9258 [Print] United States
• PMID: 8144525 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Differentiation, T-Lymphocyte
• DNA Primers
• Histocompatibility Antigens
• Membrane Glycoproteins
• ADP Ribose Transferases
• Poly(ADP-ribose) Polymerases
• Art2b protein, rat
• NAD+ Nucleosidase

Topics

• ADP Ribose Transferases
• Adenocarcinoma (enzymology)
• Amino Acid Sequence
• Animals
• Antigens, Differentiation, T-Lymphocyte (metabolism)
• Base Sequence
• Cell Line, Transformed
• DNA Primers
• Histocompatibility Antigens (biosynthesis, genetics, metabolism)
• Mammary Neoplasms, Experimental (enzymology)
• Membrane Glycoproteins
• Molecular Sequence Data
• Molecular Weight
• Muscles (enzymology)
• NAD+ Nucleosidase (biosynthesis, genetics, isolation & purification)
• Poly(ADP-ribose) Polymerases (genetics)
• Rabbits
• Rats
• Sequence Homology, Amino Acid
• Transfection
• Tumor Cells, Cultured