Abstract |
1. The major phospholipase A has been purified to electrophoretic homogeneity from the venom of Vipera russelli (Russell's viper). 2. The molecular weight of the purified enzyme was estimated to be 31,000 by Sephadex G-75 gel filtration chromatography and 29,000 by SDS- polyacrylamide gel electrophoresis. The enzyme exhibited an apparent Km value of 2.3 x 10(-2) M. 3. The phospholipase A showed edema forming, indirect hemolytic and myonecrotic activities but not hemorrhagic activity.
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Authors | Aye-Kyaw, San-Aye, Tin-Win, Hla-Pe, Mya-Maung |
Journal | The International journal of biochemistry
(Int J Biochem)
Vol. 26
Issue 1
Pg. 79-83
(Jan 1994)
ISSN: 0020-711X [Print] England |
PMID | 8138052
(Publication Type: Journal Article)
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Chemical References |
- Viper Venoms
- Phospholipases A
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Topics |
- Animals
- Edema
(chemically induced)
- Hemolysis
(drug effects)
- Hemorrhage
- Mice
- Molecular Weight
- Necrosis
- Phospholipases A
(chemistry, isolation & purification, toxicity)
- Viper Venoms
(enzymology)
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