Transformation of episodic
migraine to
chronic daily headache (so called transformed
migraine) is now a well recognized phenomenon. Although several factors, i.e.
analgesic overuse, increasing age,
psychiatric disorders are reported to play some roles in this transformation, the precise cascade is still unclear. Further suppression of an already abnormal antinociceptive system with up-regulation of post-
synaptic receptors is one of the possible explanation. In order to understand the mechanism underlying this condition,
5-HT2 serotonin receptors on platelets were assayed by the radioligand binding technique. Six transformed
migraine patients (67.67 +/- 1.52 years) and seven healthy controls (72.86 +/- 1.82 years) were studied. [3H]-
spiperone and
ketanserin were used to determine the specific binding. We found a significant increase (P < 0.05) in the maximal receptor numbers (Bmax) on platelet membrane of the
migraine patients when compared to the controls (64.31 +/- 11.06 and 39.96 +/- 5.42 fmol/mg
protein, respectively), whereas the dissociation equilibrium constant (KD) values remained unchanged (3.63 +/- 0.78 nM and 2.84 +/- 0.48 nM for the
migraine patients and controls, respectively). The up-regulation of
serotonin receptors found in this study provided further support to the "serotonergic hypofunction" theory of
migraine pathogenesis and may explain the unusual loss of episodicity seen in the transformed
migraine patients.