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[Serodiagnosis of Pseudomonas aeruginosa infections in mucoviscidosis: comparative study of Western blotting, ELISA exotoxin A and ELISA phospholipase C].

Abstract
Anti-Pseudomonas aeruginosa antibodies were studied by Western Blot, ELISA-exotoxin A and ELISA-phospholipase C for 91 serums from 31 patients with cystic fibrosis. More, for the two enzyme-linked immunosorbent assays, 44 serums from 44 healthy individuals were studied as controls. The study of these three parameters revealed the followings: with no infection by Pseudomonas aeruginosa all the results were negative, at the beginning of the infection, anti-exotoxin A antibodies appeared in first, followed in some cases by the reactions of Western Blot, anti-phospholipase C antibodies became positive at last and went on a par with the installation of the chronic characteristic of the infection, as soon as the chronicity were indisputable, the three methods revealed elevated serum antibodies amounts. Generally there was a correlation between detected antibodies and Pseudomonas aeruginosa isolation in sputum. Among these three methods, ELISA-exotoxin A appeared to be the most interesting because of its good reproducibility and its early positivity, before the others methods and sometimes before Pseudomonas aeruginosa isolation. It would be a significant argument to establish as soon as possible an antimicrobial therapy.
AuthorsC Fournier, J Andre, H Marlier, R Guinet, R Gilly
JournalPathologie-biologie (Pathol Biol (Paris)) Vol. 41 Issue 9 Pg. 856-64 (Nov 1993) ISSN: 0369-8114 [Print] France
Vernacular TitleSérodiagnostic des infections à Pseudomonas aeruginosa dans la mucoviscidose: étude comparative du Western Blot, de l'ELISA exotoxine A et de l'ELISA phospholipase C.
PMID8121717 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Bacterial Toxins
  • Exotoxins
  • Virulence Factors
  • ADP Ribose Transferases
  • Pseudomonas aeruginosa exotoxin A
  • Type C Phospholipases
Topics
  • ADP Ribose Transferases
  • Adolescent
  • Bacterial Toxins
  • Blotting, Western
  • Child
  • Child, Preschool
  • Cystic Fibrosis (diagnosis, immunology, microbiology)
  • Enzyme-Linked Immunosorbent Assay
  • Exotoxins (immunology)
  • Female
  • Humans
  • Infant
  • Male
  • Pseudomonas Infections (diagnosis, immunology, microbiology)
  • Pseudomonas aeruginosa (immunology, isolation & purification)
  • Serologic Tests
  • Staphylococcal Infections (diagnosis, microbiology)
  • Staphylococcus aureus (isolation & purification)
  • Type C Phospholipases (immunology)
  • Virulence Factors

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