Abstract |
Anti-Pseudomonas aeruginosa antibodies were studied by Western Blot, ELISA- exotoxin A and ELISA- phospholipase C for 91 serums from 31 patients with cystic fibrosis. More, for the two enzyme-linked immunosorbent assays, 44 serums from 44 healthy individuals were studied as controls. The study of these three parameters revealed the followings: with no infection by Pseudomonas aeruginosa all the results were negative, at the beginning of the infection, anti- exotoxin A antibodies appeared in first, followed in some cases by the reactions of Western Blot, anti- phospholipase C antibodies became positive at last and went on a par with the installation of the chronic characteristic of the infection, as soon as the chronicity were indisputable, the three methods revealed elevated serum antibodies amounts. Generally there was a correlation between detected antibodies and Pseudomonas aeruginosa isolation in sputum. Among these three methods, ELISA- exotoxin A appeared to be the most interesting because of its good reproducibility and its early positivity, before the others methods and sometimes before Pseudomonas aeruginosa isolation. It would be a significant argument to establish as soon as possible an antimicrobial therapy.
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Authors | C Fournier, J Andre, H Marlier, R Guinet, R Gilly |
Journal | Pathologie-biologie
(Pathol Biol (Paris))
Vol. 41
Issue 9
Pg. 856-64
(Nov 1993)
ISSN: 0369-8114 [Print] France |
Vernacular Title | Sérodiagnostic des infections à Pseudomonas aeruginosa dans la mucoviscidose: étude comparative du Western Blot, de l'ELISA exotoxine A et de l'ELISA phospholipase C. |
PMID | 8121717
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Bacterial Toxins
- Exotoxins
- Virulence Factors
- ADP Ribose Transferases
- Pseudomonas aeruginosa exotoxin A
- Type C Phospholipases
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Topics |
- ADP Ribose Transferases
- Adolescent
- Bacterial Toxins
- Blotting, Western
- Child
- Child, Preschool
- Cystic Fibrosis
(diagnosis, immunology, microbiology)
- Enzyme-Linked Immunosorbent Assay
- Exotoxins
(immunology)
- Female
- Humans
- Infant
- Male
- Pseudomonas Infections
(diagnosis, immunology, microbiology)
- Pseudomonas aeruginosa
(immunology, isolation & purification)
- Serologic Tests
- Staphylococcal Infections
(diagnosis, microbiology)
- Staphylococcus aureus
(isolation & purification)
- Type C Phospholipases
(immunology)
- Virulence Factors
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