Stabilization of the antigenicity of coxsackie B procapsids by disoxaril.

Abstract

Disoxaril added to cell cultures infected with coxsackie B virus serotypes 3 or 4 increased the yield and stabilized the antigenicity of the procapsids. In the absence of disoxaril, the procapsids lost their broad antigenicity after a short period of time. Prevention of this loss by disoxaril was observed in radioimmunoassays of IgM in sera from patients infected with various enteroviruses. The procapsids are useful in serological diagnosis of enterovirus infections, and the addition of disoxaril facilitates this technique.

• Authors: G Frisk, M Halloran, H Didarholm
• Journal: Journal of virological methods (J Virol Methods) Vol. 45 Issue 2 Pg. 235-9 (Dec 15 1993) ISSN: 0166-0934 [Print] Netherlands
• PMID: 8113348 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Immunoglobulin M
• Isoxazoles
• disoxaril

Topics

• Antibodies, Viral (blood)
• Antigens, Viral (drug effects)
• Capsid (drug effects, immunology)
• Drug Stability
• Enterovirus (immunology)
• Enterovirus B, Human (drug effects, immunology)
• Enterovirus Infections (diagnosis, immunology)
• Humans
• Immunoglobulin M (blood)
• Isoxazoles (pharmacology)
• Proviruses (drug effects, immunology)
• Radioimmunoassay (methods)
• Virology (methods)