Prevention of CD18-mediated reperfusion injury enhances the efficacy of UW solution for 15-hr heart preservation.

Abstract

University of Wisconsin solution (UW) has been demonstrated to improve donor heart preservation. During reperfusion, however, myocardial performance may still remain limited by neutrophil (PMN)-mediated injury, manifested by the "low-reflow" phenomenon and myocardial "stunning." Since PMN adhesion is an important mechanism of PMN-mediated injury, we tested whether blocking PMN adhesion molecule CD18 would enhance the efficacy of UW for 15-hr heart preservation. Rabbit hearts (n = 15) were arrested with, and preserved for 15 hr in, 4 degrees C UW. After insertion of an left ventricular (LV) balloon, hearts were heterotopically transplanted into recipients pretreated with either intravenous (i.v.) saline (vehicle, n = 8) or i.v. anti-CD18 monoclonal antibody R15.7 (anti-CD18, n = 7). After 1 hr reperfusion the slope of the peak systolic pressure-volume (P-V) relation (Emax), the exponential coefficient (beta), the volume-axis intercept (Vo) of the end-diastolic P-V relation, and the time constant of the exponential LV pressure decay after dP/dt min (tau) were measured. Blood flow was measured with microspheres from which coronary vascular resistance (CVR) was calculated. Tissue %H2O was also measured. Between groups there were no significant differences in Emax (70.5 +/- 6.1 vs 63.7 +/- 6.0 mm Hg/ml, P > 0.05), beta (3.3 +/- 0.5 vs 4.3 +/- 0.5, P > 0.05), Vo (-0.4 +/- 0.1 vs -0.4 +/- 0.2 ml, P > 0.05) or %H2O (81.3 +/- 1.1 vs 80.0 +/- 0.9, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: J G Byrne, M P Murphy, W J Smith, G S Couper, R F Appleyard, L H Cohn
• Journal: The Journal of surgical research (J Surg Res) Vol. 54 Issue 6 Pg. 625-30 (Jun 1993) ISSN: 0022-4804 [Print] United States
• PMID: 8105148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• CD18 Antigens
• Insulin
• Organ Preservation Solutions
• University of Wisconsin-lactobionate solution
• Allopurinol
• Adenosine Triphosphate
• Glutathione
• Adenosine
• Raffinose

Topics

• Adenosine (pharmacology)
• Adenosine Triphosphate (metabolism)
• Allopurinol (pharmacology)
• Animals
• Antigens, CD (physiology)
• CD18 Antigens
• Glutathione (pharmacology)
• Heart Transplantation
• Insulin (pharmacology)
• Myocardial Reperfusion Injury (prevention & control)
• Neutrophils (physiology)
• Organ Preservation
• Organ Preservation Solutions
• Rabbits
• Raffinose (pharmacology)