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Participation of serum proteins in the inflammation-primed activation of macrophages.

Abstract
Inflamed lesions release degradation products of membrane lipids, lysophospholipids, and inflamed tumor tissues release alkylglycerols. Macrophages were activated by administration of lysophosphatidylcholine (lyso-Pc) or dodecylglycerol (DDG) to mice. In vitro treatment of mouse peritoneal cells (mixture of nonadherent and adherent cells) with lyso-Pc or DDG in fetal calf serum supplemented medium for 30 min, followed by 3-h cultivation of adherent cells (macrophages) alone, resulted in greatly enhanced Fc-receptor mediated phagocytic activity and superoxide generating capacity of macrophages. The tumor lipid metabolite, DDG, is far more potent (400-fold) than lyso-Pc in terms of doses required for the maximal levels of macrophage activation. The inflammation-primed macrophage activation required a serum factor, vitamin D binding protein, as a precursor for the macrophage activating factor. Treatment of mouse peritoneal cells with 1 microgram lyso-Pc/ml or 50 ng DDG/ml in a serum-free 0.1% egg albumin supplemented medium for 30 min, followed by 3-h cultivation of the treated peritoneal cells in a medium supplemented with a very small amount (0.0005-0.05%) of ammonium sulfate [20-50% saturated (NH4)2SO4] precipitable protein fraction of FCS, resulted in greatly enhanced superoxide generating capacity of macrophages. The ammonium sulfate precipitable fraction was found to contain vitamin D binding protein.
AuthorsN Yamamoto, N P Willett, D D Lindsay
JournalInflammation (Inflammation) Vol. 18 Issue 3 Pg. 311-22 (Jun 1994) ISSN: 0360-3997 [Print] United States
PMID8088927 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Blood Proteins
  • Glycerides
  • Laurates
  • Lysophosphatidylcholines
  • Monoglycerides
  • Superoxides
  • monolaurin
  • Ammonium Sulfate
Topics
  • Ammonium Sulfate (pharmacology)
  • Animals
  • Blood Proteins (physiology)
  • Cattle (embryology)
  • Chemical Fractionation
  • Dose-Response Relationship, Drug
  • Female
  • Fetal Blood (physiology)
  • Glycerides (pharmacology)
  • Inflammation (pathology, physiopathology)
  • Laurates (pharmacology)
  • Lysophosphatidylcholines (pharmacology)
  • Macrophages, Peritoneal (physiology)
  • Mice
  • Mice, Inbred BALB C
  • Monoglycerides
  • Osmolar Concentration
  • Superoxides (metabolism)

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