Abstract |
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80% to 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100%, 85%, and 65%, respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.
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Authors | J F Lindo, D J Conway, N S Atkins, A E Bianco, R D Robinson, D A Bundy |
Journal | The American journal of tropical medicine and hygiene
(Am J Trop Med Hyg)
Vol. 51
Issue 2
Pg. 175-9
(Aug 1994)
ISSN: 0002-9637 [Print] United States |
PMID | 8074251
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Immunoglobulin G
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Topics |
- Animals
- Antibodies, Helminth
(blood)
- Antigens, Helminth
(immunology)
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay
- Evaluation Studies as Topic
- False Positive Reactions
- Feces
(parasitology)
- Humans
- Immunoblotting
- Immunoglobulin G
(blood)
- Larva
(immunology)
- Onchocerca
(immunology)
- Prospective Studies
- Sensitivity and Specificity
- Strongyloides stercoralis
(immunology)
- Strongyloidiasis
(diagnosis)
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