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Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection.

Abstract
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80% to 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100%, 85%, and 65%, respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.
AuthorsJ F Lindo, D J Conway, N S Atkins, A E Bianco, R D Robinson, D A Bundy
JournalThe American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 51 Issue 2 Pg. 175-9 (Aug 1994) ISSN: 0002-9637 [Print] United States
PMID8074251 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Helminth
  • Antigens, Helminth
  • Immunoglobulin G
Topics
  • Animals
  • Antibodies, Helminth (blood)
  • Antigens, Helminth (immunology)
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • False Positive Reactions
  • Feces (parasitology)
  • Humans
  • Immunoblotting
  • Immunoglobulin G (blood)
  • Larva (immunology)
  • Onchocerca (immunology)
  • Prospective Studies
  • Sensitivity and Specificity
  • Strongyloides stercoralis (immunology)
  • Strongyloidiasis (diagnosis)

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