Abstract |
Steroid sulfatase (STS) desulfates a number of 3 beta-hydroxysteroid sulfates, converting inactive steroid hormone to the active form. We have established an enzyme-linked immunosorbent assay (ELISA) of STS by using polyclonal antibody against STS purified from human placenta to measure the amount of the enzyme protein in sera. ELISA was performed by a 'Sandwich' method using a peroxidase conjugated anti-STS IgG Fab' fragment. A range of STS of 10-1,500 ng/ml in serum was assayed by this method. When the serum STS from the patients with gynecologic carcinomas was assayed by the ELISA, the level was significantly elevated in endometrial carcinoma (P < 0.05) and ovarian carcinoma (P < 0.01), respectively, as compared with that of normal healthy women.
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Authors | T Sugawara, K Honke, S Gasa, T Tanaka, S Fujimoto, A Makita |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 226
Issue 1
Pg. 13-20
(Apr 1994)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 8070131
(Publication Type: Journal Article)
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Chemical References |
- Immunoglobulin Fab Fragments
- Arylsulfatases
- Steryl-Sulfatase
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Topics |
- Adult
- Aged
- Aging
(blood)
- Animals
- Arylsulfatases
(blood, immunology, isolation & purification)
- Blotting, Western
- Carcinoma
(enzymology)
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Genital Neoplasms, Female
(enzymology)
- Humans
- Immunoglobulin Fab Fragments
- Male
- Middle Aged
- Rabbits
- Reference Values
- Steryl-Sulfatase
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