Abstract | OBJECTIVES: To evaluate the effects of different surfactants on pulmonary infection with group B streptococci in premature rabbits and to examine the effects of different surfactants on pulmonary alveolar macrophage function of newborn rabbits. MODEL: Preterm and term rabbit pups. METHODS: RESULTS: Preterm animals had a sixfold increase in pulmonary bacterial growth compared with a slight decrease in intrapulmonary GBS in term animals when all were delivered by cesarean section (p < 0.05). In premature rabbits, GBS proliferation was lowest in animals treated with Exosurf Neonatal and highest in animals receiving Curosurf and human amniotic fluid-derived surfactant (p < 0.05). None of the surfactants promoted accelerated growth of GBS in comparison with control animals. Similar growth of GBS was seen in in vitro cultures. Intrapulmonary phagocytosis of GBS in premature pups was not altered by any of the surfactants. In term rabbit pups, the following measures of macrophage population kinetics remained normal at 1 and 24 hours after surfactant administration: viability, cell numbers based on lung lavage, and in vivo incorporation of thymidine. CONCLUSIONS:
Surfactants used in clinical practice do not accelerate the in vivo growth of group B streptococci in the lungs of preterm rabbits. Some surfactants inhibit streptococcal proliferation. The effects of different surfactants are not explained by changes in macrophage function.
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Authors | M P Sherman, L A Campbell, T A Merritt, W A Long, J H Gunkel, T Curstedt, B Robertson |
Journal | The Journal of pediatrics
(J Pediatr)
Vol. 125
Issue 6 Pt 1
Pg. 939-47
(Dec 1994)
ISSN: 0022-3476 [Print] United States |
PMID | 7996369
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Biological Products
- Drug Combinations
- Fatty Alcohols
- Phospholipids
- Pulmonary Surfactants
- Phosphorylcholine
- Polyethylene Glycols
- dipalmitoylphosphatidylcholine, hexadecanol, tyloxapol drug combination
- poractant alfa
- beractant
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Topics |
- Animals
- Animals, Newborn
- Biological Products
- Cell Division
(drug effects)
- Drug Combinations
- Fatty Alcohols
(pharmacology, therapeutic use)
- Lung Diseases
(drug therapy, microbiology)
- Macrophages, Alveolar
(drug effects, microbiology, pathology)
- Models, Biological
- Phagocytosis
(drug effects)
- Phospholipids
- Phosphorylcholine
- Polyethylene Glycols
(pharmacology, therapeutic use)
- Pulmonary Surfactants
(pharmacology, therapeutic use)
- Rabbits
- Streptococcal Infections
(drug therapy, microbiology)
- Streptococcus agalactiae
(cytology, drug effects, growth & development, isolation & purification)
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