Urinary excretion of 2,3-dinor-thromboxane B1, a major metabolite of thromboxane B2 in the rat.

Urinary 2,3-dinor-thromboxane B2 (2,3-dinor-TXB2), an enzymatic degradation product of TXB2, is currently measured for evaluating in vivo thromboxane biosynthesis in rats. We simultaneously measured 2,3-dinor-TXB2 and 2,3-dinor-TXB1, another product of TXB2 metabolism, in the urine of rats by immunoaffinity extraction/gas chromatography negative ion chemical ionization mass spectrometry (GC-NICIMS). In rats under basal conditions, urinary excretion of 2,3-dinor-TXB1 was much higher than that of 2,3-dinor-TXB2 (19.22 +/- 4.86 and 1.64 +/- 0.29 ng/24 h, respectively). The relative abundance of the two metabolites in each animal was fairly constant (91.9 +/- 1.6 and 8.1 +/- 1.6% of their sum, respectively). Urinary excretion of both 2,3-dinor-TXB1 and 2,3-dinor-TXB2 increased in rats undergoing in vivo hepatic ischemia-reperfusion. Other thromboxane metabolites, including 11-dehydro-TXB2 and 11-dehydro-2,3-dinor-TXB2, were measured by GC-NICIMS in selected urines. The resulting profile was: 2,3,4,5-tetranor-TXB1 > 2,3-dinor-TXB1 >> 11-dehydro-TXB2 > 2,3-dinor-TXB2 = TXB2. This study shows that urinary 2,3-dinor-TXB1 is a suitable parameter of TXB2 biosynthesis in vivo in rats. The possible cross-reactivity of 2,3-dinor-TXB1 in immunoassays of urinary 2,3-dinor-TXB2 or even TXB2 in rats should be considered in future studies.
AuthorsC Chiabrando, M Corada, A Bachi, R Fanelli
JournalProstaglandins (Prostaglandins) Vol. 47 Issue 6 Pg. 409-22 (Jun 1994) ISSN: 0090-6980 [Print] UNITED STATES
PMID7938613 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 2,3,4,5-tetranorthromboxane B1
  • 2,3-dinorthromboxane B1
  • Thromboxanes
  • 11-dehydro-2,3-dinor-thromboxane B2
  • Thromboxane B2
  • 2,3-dinor-thromboxane B2
  • 11-dehydro-thromboxane B2
  • Animals
  • Ischemia
  • Liver (blood supply)
  • Male
  • Mass Spectrometry
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion
  • Thromboxane B2 (analogs & derivatives, isolation & purification, metabolism, urine)
  • Thromboxanes (analogs & derivatives)

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