Abstract |
A vincristine (Vcr)-resistant subline of the human histiocytic lymphoma cell line U-937 (U-937-vcr) has been established and characterized with respect to its phenotypic features, including growth rate, surface marker expression and ability to respond to differentiation-inducing agents. The sensitivity of U-937-vcr cells to the direct cytotoxicity of cyclosporin A (CsA) and verapamil (Ver), and the capacity of these drugs to modify Vcr resistance, were also examined. The U-937-vcr cells exhibited a more than 200-fold resistance to Vcr, and cross-resistance to vinorelbin and taxol. Also, there was a slight cross-resistance to colchicine, doxorubicin and VP16. However, the response of U-937-vcr to CsA or Ver alone was substantially altered, with a marked decrease in their respective IC50s. The U-937-vcr cells did not show increased levels of pgp 170. We conclude that the development of Vcr resistance was not associated with a change in the major phenotypic properties of the U-937 cell line, and that resistance modifier hypersensitivity was not associated with increase in pgp 170 expression.
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Authors | J Botling, G Liminga, R Larsson, P Nygren, K Nilsson |
Journal | International journal of cancer
(Int J Cancer)
Vol. 58
Issue 2
Pg. 269-74
(Jul 15 1994)
ISSN: 0020-7136 [Print] United States |
PMID | 7913083
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Antigens, Surface
- Carrier Proteins
- Membrane Glycoproteins
- Vincristine
- Cyclosporine
- Verapamil
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Topics |
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Antigens, Surface
(physiology)
- Carrier Proteins
(physiology)
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Cyclosporine
(pharmacology)
- Drug Interactions
- Drug Resistance
- Drug Screening Assays, Antitumor
- Humans
- Lymphoma, Large B-Cell, Diffuse
(drug therapy, pathology)
- Membrane Glycoproteins
(physiology)
- Tumor Cells, Cultured
- Verapamil
(pharmacology)
- Vincristine
(pharmacology)
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