Abstract |
We have used antisense phosphorothioate oligonucleotides to define the role played by proliferating cell nuclear antigen ( PCNA) in neointimal accumulation of smooth muscle cells in a rat carotid artery injury model. The short-term extraluminal delivery of 250 nmol of antisense oligonucleotides, but not control oligonucleotides, immediately after arterial injury produces a 77% suppression of PCNA mRNA after 24 h and a 52% decrease in the frequency of medial smooth muscle cells expressing PCNA after 72 h. This reduction in PCNA expression is accompanied by a 59% decrease in the frequency of proliferating medial smooth muscle cells at 3 d as measured by BudR staining and an 80% decrease in neointimal accumulation assessed morphometrically at 2 wk. Thus, the expression of PCNA is required for medial smooth muscle cell growth in vivo and for neointimal formation after arterial injury.
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Authors | M Simons, E R Edelman, R D Rosenberg |
Journal | The Journal of clinical investigation
(J Clin Invest)
Vol. 93
Issue 6
Pg. 2351-6
(Jun 1994)
ISSN: 0021-9738 [Print] United States |
PMID | 7911126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Nuclear Proteins
- Oligonucleotides, Antisense
- Proliferating Cell Nuclear Antigen
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Topics |
- Animals
- Base Sequence
- Carotid Arteries
(pathology)
- Cell Division
- Cell Line
- Hyperplasia
- Molecular Sequence Data
- Muscle, Smooth, Vascular
(pathology)
- Nuclear Proteins
(analysis, physiology)
- Oligonucleotides, Antisense
(pharmacology)
- Proliferating Cell Nuclear Antigen
- Rats
- Rats, Sprague-Dawley
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