Abstract |
Human renal epithelial and mesangial cells have been shown to synthesise complement C3 in culture, but the relevance of this finding to the development of complement-mediated nephritis is uncertain. We investigated C3 gene expression in tissue biopsies that showed three main categories of renal injury. By semiquantitative polymerase chain reaction, biopsies from patients with immune-complex glomerulonephritis and those with cell-mediated interstitial nephritis showed increased C3 expression (p < 0.05), but biopsies from patients with non-immune glomerular injury did not. These findings suggest that local C3 production is enhanced in immune-mediated nephritis and are consistent with the hypothesis that locally synthesised complement components are involved in the pathogenesis of tissue injury.
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Authors | S H Sacks, W Zhou, P A Andrews, B Hartley |
Journal | Lancet (London, England)
(Lancet)
Vol. 342
Issue 8882
Pg. 1273-4
(Nov 20 1993)
ISSN: 0140-6736 [Print] England |
PMID | 7901586
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Complement C3c
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Topics |
- Complement C3c
(biosynthesis, genetics)
- Culture Techniques
- Gene Expression
- Humans
- Immune Complex Diseases
(genetics, immunology, metabolism)
- Kidney Cortex
(immunology, metabolism)
- Nephritis
(genetics, immunology, metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
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