Abstract | BACKGROUND/AIMS: Vesicular transport is supported by microtubule-based, force-transducing adenosine triphosphatases ( ATPases), such as kinesin, a ubiquitous motor enzyme that has been well studied in neuronal tissues. Although vesicular transport is important for hepatocellular secretory and clearance activities, the role of kinesin in liver function is poorly understood. Furthermore, the effects of bile acids on kinesin are unknown. METHODS:
Kinesin was purified from rat liver cytosol by conventional chromatography and microtubule affinity binding and was characterized by immunoblotting with domain-specific kinesin antibodies and amino acid sequencing of tryptic fragments. Kinesin activity was measured with and without bile acids using an in vitro motility assay and ATPase assays. RESULTS: CONCLUSIONS: Cholestatic concentrations of chenodeoxycholate conjugates directly inhibit the activity of microtubule motors, suggesting a possible mechanism for impairment of vesicular transport in cholestasis.
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Authors | D L Marks, N F LaRusso, M A McNiven |
Journal | Gastroenterology
(Gastroenterology)
Vol. 108
Issue 3
Pg. 824-33
(Mar 1995)
ISSN: 0016-5085 [Print] United States |
PMID | 7875485
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bile Acids and Salts
- Chenodeoxycholic Acid
- Taurochenodeoxycholic Acid
- Kinesins
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Topics |
- Amino Acid Sequence
- Animals
- Bile Acids and Salts
(pharmacology)
- Chenodeoxycholic Acid
(pharmacology)
- Cholestasis
(metabolism)
- Kinesins
(antagonists & inhibitors, genetics, isolation & purification)
- Liver
(metabolism)
- Microtubules
(metabolism)
- Molecular Sequence Data
- Rats
- Rats, Sprague-Dawley
- Taurochenodeoxycholic Acid
(pharmacology)
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