Abstract |
We have developed a rapid and simple method to detect the relation between HLA-DQ beta 57 Asp and Chinese IDDM patients. The method involved the selective amplification of a DNA fragment from the HLA-DQ B1 gene by using the mutagenic primers. After PCR, if the HLA-DQ beta 57 was Asp, then there was an artificially created restriction enzyme cutting site. We then can accurately obtain the results by enzyme digestion and electrophoresis. Sixty-nine IDDM patients and 30 nondiabetic control subjects were analyzed using this method. Twenty-two (42%) IDDM patients had non-Asp 57 homozygous, 31/45%) were Asp/non-Asp 57 heterozygous, and 9 (13%) had Asp-57 homozygous. Of the 30 control subjects, the number of cases for these three types were 6 (20%), 18 (60%), and 6 (20%), respectively. The relative risk of homozygous DQ beta 57 non-Asp in our group was 2.9 and the p value was greater than 0.05. Using this kind of approach, we were able to provide a simple, rapid, and non-radioactive method to detect whether the HLA DQ beta 57 was Asp or not.
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Authors | Y W Huang, L S Lee, M C Shih, Y H Pai, Y J Lee, J G Chang |
Journal | Tissue antigens
(Tissue Antigens)
Vol. 44
Issue 3
Pg. 155-8
(Sep 1994)
ISSN: 0001-2815 [Print] England |
PMID | 7839347
(Publication Type: Journal Article)
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Chemical References |
- Genetic Markers
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- HLA-DQbeta antigen
- DNA Restriction Enzymes
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Topics |
- Adolescent
- Aged
- Base Sequence
- Child
- DNA Restriction Enzymes
- Diabetes Mellitus, Type 1
(genetics)
- Female
- Genetic Markers
- HLA-DQ Antigens
(genetics)
- HLA-DQ beta-Chains
- Humans
- Male
- Middle Aged
- Molecular Sequence Data
- Polymerase Chain Reaction
(methods)
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