Local immunie response in experimental pyelonephritis in the rabbit. III. Lymphocyte responsivieness to O and K antigens of Escherichia coli.

Abstract

The response of circulating and infected kidney lymphocytes to the O (lipopolysaccharide) and K (polysaccharide) antigens of an Escherichia coli O6 K 13 H1 strain was determined. Both circulating and kidney lymphocytes showed significant incorporation of [3H-methyl]thymidine into DNA when incubated with the O antigen, whereas neither responded to the K antigen. The lipid moiety of the lipopolysaccharide was required for lymphocyte responsiveness. Upon sequential incubation of O antigen and fluoresceinated homologous antiserum, 24-30 per cent of kidney lymphocytes were shown to have surface receptors for O antigen, whereas none had surface receptors for K antigen. Although the K antigen is an important determinant of invasiveness of the upper urinary tract, it fails to elicit a cellular immune response or attach to lymphocytes from the infected kidney in experimental pyelonephritis.

• Authors: J W Smith, B Kaijser
• Journal: Immunology (Immunology) Vol. 31 Issue 2 Pg. 233-7 (Aug 1976) ISSN: 0019-2805 [Print] England
• PMID: 783043 (Publication Type: Journal Article)

Chemical References

• Antigens, Bacterial
• Lipopolysaccharides
• Polysaccharides, Bacterial
• DNA

Topics

• Animals
• Antibody Formation
• Antigens, Bacterial
• DNA (biosynthesis)
• Escherichia coli (immunology)
• Fluorescent Antibody Technique
• Immunity, Cellular
• Lipopolysaccharides (immunology)
• Lymphocyte Activation
• Lymphocytes (immunology)
• Polysaccharides, Bacterial (immunology)
• Pyelonephritis (immunology)
• Rabbits