Effect of in vivo macrophage colony-stimulating factor on fungistasis of bronchoalveolar and peritoneal macrophages against Cryptococcus neoformans.

Abstract

Macrophage colony-stimulating factor (M-CSF) given subcutaneously at a dose of 2.5 mg/kg of body weight (4.75 x 10(6) U/kg) to CD-1 male mice 8 to 12 weeks old was found to enhance significantly the fungistasis of bronchoalveolar macrophages (BAM) against Cryptococcus neoformans. When M-CSF was given 1, 3, 7, 9, or 13 days before an ex vivo challenge with C. neoformans, fungistasis was increased (P ranged from < 0.05 to < 0.001) compared with that induced by control BAM. A maximum effect was seen by days 1 and 3 after administration of M-CSF. Twenty-one days after M-CSF, BAM did not produce significantly enhanced fungistasis. M-CSF also significantly enhances the fungistatic effect of peritoneal macrophages (PM) if given 1, 3, and 7 days prior to testing against C. neoformans in comparison with control PM (P ranged from < 0.05 to < 0.001). PM did not produce enhanced fungistasis 9 or 13 days after administration of M-CSF. These studies demonstrating in vivo enhancement of anticryptococcal activity of macrophages with M-CSF provide a rationale for in vivo use of M-CSF to enhance resistance to infection with C. neoformans.

• Authors: F Nassar, E Brummer, D A Stevens
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 38 Issue 9 Pg. 2162-4 (Sep 1994) ISSN: 0066-4804 [Print] United States
• PMID: 7811036 (Publication Type: Journal Article)

Chemical References

• Macrophage Colony-Stimulating Factor

Topics

• Animals
• Cryptococcosis (drug therapy, immunology)
• Cryptococcus neoformans (drug effects, growth & development, immunology)
• Injections, Subcutaneous
• Kinetics
• Macrophage Colony-Stimulating Factor (pharmacology)
• Macrophages, Alveolar (drug effects, microbiology)
• Macrophages, Peritoneal (drug effects, microbiology)
• Male
• Mice
• Mice, Inbred Strains