Abstract |
Using PCR method and family specific primers for T-cell receptor gamma chain V gene, the T-cell colonalities of 12 cases of AILD-TCL were analyzed. The results showed monoclonal DNA amplification patterns of TCR gamma in all 12 cases (13 specimens), whereas the DNA from normal peripheral lymphocytes and 2 samples of atypical lymphoid hyperplasia showed polyclonal rearrangement patterns. Our results suggest that AILD-TCL is a monoclonal proliferative disorder originating from T-cell. The PCR method and family specific primers could successfully amplicate the template DNA from paraffin sections, so it is a sensitive, simple and rapid method for routine hematopathologic studies.
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Authors | G D Li, J Lorenzen, M L Hansmann |
Journal | Zhonghua bing li xue za zhi = Chinese journal of pathology
(Zhonghua Bing Li Xue Za Zhi)
Vol. 23
Issue 4
Pg. 211-4
(Aug 1994)
ISSN: 0529-5807 [Print] China |
PMID | 7805148
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Aged
- Base Sequence
- DNA, Neoplasm
(genetics)
- Female
- Follow-Up Studies
- Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
- Humans
- Immunoblastic Lymphadenopathy
(genetics)
- Lymphoma, T-Cell
(genetics)
- Male
- Middle Aged
- Molecular Sequence Data
- Polymerase Chain Reaction
- Prognosis
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