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Application of 'nose-poke habituation' validation with post-trial diazepam- and cholecystokinin-induced hypo- and hypermnesia.

Abstract
The present study describes the use of nose-poke habituation as a memory task and demonstrates that it is sensitive to hypo- and hypermnestic pharmacological treatments administered post-trial. Habituation of nose-poke behavior of rats was defined as a reduction in number of nose-pokes compared to baseline. It was measured using a board with 16 holes, to which animals were exposed on 2 consecutive days (baseline and test) for 10 min, respectively. After the first exposure, rats were injected intraperitoneally (i.p.) immediately or with a delay of 2.5 h with doses of diazepam (0.9-4.5 mg/kg) known to be hypomnestic, or cholecystokinin (CCK-8S; 0.2-25 micrograms/kg), which was reported to have memory facilitating effects. An enhancement of habituation in comparison with vehicle controls was interpreted in terms of a hypermnestic effect of the treatment. Conversely, hypomnestic action of the drug treatment was inferred from a reduced habituation. The results show that when diazepam was injected immediately post-trial, the normal reduction in number of nose-pokes during test was prevented, indicative of a failure to habituate presumably due to an amnesia for the baseline/training trial. In contrast, enhanced habituation (facilitation of memory) was induced when CCK-8S was injected immediately post-trial, as reflected by a decrease in number of nose-pokes during test compared to control animals. The effects were not due to enduring proactive effects of the compounds on performance during test, since post-trial injections of diazepam or CCK-8S with a delay of 2.5 h did not have the effects that immediate post-trial injection had.(ABSTRACT TRUNCATED AT 250 WORDS)
AuthorsM Voits, H Fink, P Gerhardt, J P Huston
JournalJournal of neuroscience methods (J Neurosci Methods) Vol. 57 Issue 1 Pg. 101-5 (Mar 1995) ISSN: 0165-0270 [Print] Netherlands
PMID7791360 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cholecystokinin
  • Diazepam
Topics
  • Animals
  • Cholecystokinin (administration & dosage, pharmacology)
  • Diazepam (administration & dosage, pharmacology)
  • Dose-Response Relationship, Drug
  • Habituation, Psychophysiologic (drug effects)
  • Injections, Intraperitoneal
  • Male
  • Memory (drug effects)
  • Rats
  • Rats, Wistar

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