Differential mechanism of retention of Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) (Cu-PTSM) by brain and tumor: a novel radiopharmaceutical for positron emission tomography imaging.

Abstract

The reductive retention of 62Cu-PTSM was comparatively studied in the brain and Ehrlich ascites tumor cells by electron spin resonance spectrometry and nonradioactive Cu-PTSM. In the brain, only the mitochondrial fraction showed the ability to reduce Cu-PTSM, and the other subcellular fractions did not. In contrast, the cytosolic fraction of Ehrlich ascites tumor cells was the specific site of Cu-PTSM reduction. It was therefore considered that the retention of Cu-PTSM in the brain is closely related to mitochondrial reduction, most probably involving the mitochondrial electron transport system.

• Authors: Y Fujibayashi, H Taniuchi, K Wada, Y Yonekura, J Konishi, A Yokoyama
• Journal: Annals of nuclear medicine (Ann Nucl Med) Vol. 9 Issue 1 Pg. 1-5 (Feb 1995) ISSN: 0914-7187 [Print] Japan
• PMID: 7779524 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Organometallic Compounds
• Thiosemicarbazones
• copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex
• Copper
• L-Lactate Dehydrogenase
• Succinate Dehydrogenase
• NADH Dehydrogenase

Topics

• Animals
• Biomarkers (analysis)
• Brain (diagnostic imaging, metabolism)
• Carcinoma, Ehrlich Tumor (diagnostic imaging, metabolism)
• Cell Nucleus (diagnostic imaging, metabolism)
• Copper (pharmacokinetics)
• Cytosol (diagnostic imaging, metabolism)
• Electron Spin Resonance Spectroscopy
• L-Lactate Dehydrogenase (analysis)
• Mice
• Microsomes (diagnostic imaging, metabolism)
• Mitochondria (diagnostic imaging, metabolism)
• NADH Dehydrogenase (analysis)
• Organometallic Compounds (pharmacokinetics)
• Succinate Dehydrogenase (analysis)
• Thiosemicarbazones (pharmacokinetics)
• Tomography, Emission-Computed
• Tumor Cells, Cultured