HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Systemic immunization with Hsp60 alters the development of chlamydial ocular disease.

AbstractPURPOSE:
To determine whether immunization with recombinant Hsp60 would exacerbate ocular pathology on challenge with viable chlamydial elementary bodies.
METHODS:
Guinea pigs were immunized either subcutaneously with recombinant Hsp60 or both subcutaneously with recombinant Hsp60 and ocularly with attenuated Salmonella typhimurium expressing the guinea pig inclusion conjunctivitis (GPIC) Hsp60 antigen. All animals were challenged in the conjunctiva with the agent of GPIC, and the degree of gross ocular pathology was determined. Immunoglobulin G (IgG) and immunoglobulin A (IgA) antibody titers to Hsp60 were measured in ocular secretions as a measure of the degree of immunization.
RESULTS:
In primary and challenge GPIC infection, the degree of gross ocular pathology was lower in the immunized group. The presence of high IgA and IgG antibody titers to Hsp60 in tears suggested that the response may have been modified by the presence of blocking antibodies that either may have removed the antigen quickly or prevented interaction with sensitized T cells. In contrast to subcutaneous immunization, the combined immunization regimen, consisting of subcutaneous recombinant Hsp60 followed by ocular inoculation of the attenuated Salmonella, resulted in no difference in gross pathology after reinfection of guinea pigs with GPIC.
CONCLUSIONS:
These data indicated that the immunization with Hsp60 did not produce exacerbated disease on challenge with viable organisms; however, the data suggested that the route of administration, form of antigen, or both may be critical in the disease process.
AuthorsR G Rank, C Dascher, A K Bowlin, P M Bavoil
JournalInvestigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 36 Issue 7 Pg. 1344-51 (Jun 1995) ISSN: 0146-0404 [Print] United States
PMID7775112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Chaperonin 60
  • Immunoglobulin A
  • Immunoglobulin G
  • Recombinant Proteins
Topics
  • Animals
  • Antibodies, Bacterial (analysis)
  • Antigens, Bacterial (administration & dosage, genetics, immunology)
  • Chaperonin 60 (administration & dosage, genetics, immunology)
  • Chlamydia trachomatis (genetics, immunology)
  • Conjunctiva (immunology)
  • Conjunctivitis, Inclusion (immunology, pathology)
  • Disease Models, Animal
  • Female
  • Gene Expression Regulation, Bacterial
  • Guinea Pigs
  • Immunization
  • Immunoglobulin A (analysis)
  • Immunoglobulin G (analysis)
  • Injections, Subcutaneous
  • Recombinant Proteins (administration & dosage, genetics, immunology)
  • Salmonella typhimurium (genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: