Immunohistochemical colocalization of Met5-enkephalin-Arg6-Gly7-Leu8 with tyrosine hydroxylase in neurons of the porcine inferior mesenteric and coeliac-superior mesenteric ganglia.

Abstract

The localization and distribution of the proenkephalin A-derived octapeptide Met5-enkephalin-Arg6-Gly7-Leu8 (MEAGL) was studied in the porcine sympathetic prevertebral ganglia by using the indirect immunofluorescence technique. In the inferior mesenteric ganglion, a small population of principal neurons and SIF cells contained intense MEAGL immunoreactivity. A very dense plexus of MEAGL-immunoreactive nerve fibers was distributed in different parts of the ganglion. A low number of MEAGL- immunoreactive principal ganglion cells and SIF cells, as well as numerous nerve fibers were also present in the coeliac-superior mesenteric ganglion. Double staining experiments indicated that the MEAGL-immunoreactive principal neurons in the inferior mesenteric and coeliac-superior mesenteric ganglia also contained tyrosine hydroxylase immunoreactivity.

• Authors: M Lakomy, O Häppölä, K Wasowicz
• Journal: Folia histochemica et cytobiologica (Folia Histochem Cytobiol) Vol. 32 Issue 4 Pg. 225-30 ( 1994) ISSN: 0239-8508 [Print] Poland
• PMID: 7758615 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies
• Enkephalin, Methionine
• enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-
• Tyrosine 3-Monooxygenase

Topics

• Animals
• Antibodies (immunology)
• Enkephalin, Methionine (analogs & derivatives, analysis, immunology)
• Fluorescent Antibody Technique
• Ganglia, Sympathetic (chemistry, cytology)
• Neurons (chemistry)
• Swine
• Tyrosine 3-Monooxygenase (analysis, immunology)