Abstract | OBJECTIVE: SETTING: University-based center for reproductive medicine. DESIGN: Double-blind randomized placebo cross-over controlled trial. PATIENTS, PARTICIPANTS: INTERVENTIONS: Patients were allocated to two groups according to the blinded randomization. Each patient received either 600 mg/d of vitamin E ( Ephynal, 300 mg tablets; F. Hoffman-La Roche Ltd., Basle, Switzerland) (order A) or identical placebo tablets (order B) for 3 months. Then after a 1-month wash-out period the patients were crossed-over to the other treatment. MAIN OUTCOME MEASURES: Improvement in the in vitro function of the spermatozoa measured by conventional semen analysis, computerized motility assessment, determination of reactive oxygen species generation, binding to the zona pellucida of the unfertilized human oocyte in a competitive zona binding assay, development of hyperactivated motility (both spontaneous and in the presence of 20% of the natural agonist, human follicular fluid) and pregnancy. RESULTS: Rise in the blood serum vitamin E levels after treatment accompanied by improvement in one of the sperm function tests: the zona binding assay. The zona binding ratio for order A improved from 0.2 (range 0 to 0.5) before treatment to 0.5 (range 0.1 to 1.0) after treatment, the corresponding values for order B were 0.2 (range 0 to 1.0) before treatment and 0.3 (range 0.1 to 0.7) after treatment. CONCLUSION:
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Authors | E Kessopoulou, H J Powers, K K Sharma, M J Pearson, J M Russell, I D Cooke, C L Barratt |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 64
Issue 4
Pg. 825-31
(Oct 1995)
ISSN: 0015-0282 [Print] United States |
PMID | 7672157
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Reactive Oxygen Species
- Vitamin E
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Topics |
- Adult
- Cross-Over Studies
- Double-Blind Method
- Female
- Humans
- Infertility, Male
(drug therapy, etiology, metabolism)
- Male
- Middle Aged
- Reactive Oxygen Species
(metabolism)
- Semen
(metabolism)
- Spermatozoa
(drug effects, physiology)
- Treatment Outcome
- Vitamin E
(therapeutic use)
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