Plasma glutathione S-transferase as an early marker of posttraumatic hepatic injury in non-human primates.

Abstract

We have hypothesized that the measurement of alpha-glutathione S-transferase (alpha-GST) in serum may provide a suitable sensitive marker of shock-induced liver damage. Six male adult baboons were studied. Hemorrhage was induced by blood withdrawal of 60-70% of the total blood volume down to an arterial pressure of 35-40 mmHg. Then the reinfusion was performed with the heparinized shed blood plus the same amount of Ringer's solution over the next 4 h. Before the start of hemorrhage, 2 mL/kg zymosan-activated plasma was infused to simulate trauma-related complement activation. alpha-GST antigen levels were determined using an anti-human alpha-GST immunoassay (Hepkit). Concentrations of alpha-GST at baseline in baboon were found to be 3.1 +/- 1.8 ng/mL; at the end of the shock period a significant increase in alpha-GST serum levels (74.8 +/- 13.8 ng/mL) was found. In contrast, transaminase levels did not significantly change. From the current evidence in posttraumatic non-human primates, which resemble the clinical situation in several aspects, alpha-GST measurements are a suitable marker of early hepatocellular injury.

• Authors: H Redl, G Schlag, E Paul, J Davies
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 3 Issue 6 Pg. 395-7 (Jun 1995) ISSN: 1073-2322 [Print] United States
• PMID: 7656061 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Glutathione Transferase

Topics

• Animals
• Biomarkers
• Glutathione Transferase (blood)
• Liver (enzymology, injuries)
• Male
• Papio
• Shock (enzymology, etiology)