The aim of this study was to assess whether the presentation and progression of autoimmune
postpartum thyroiditis (PPT) was related to the degree of
thyroid peroxidase autoantibody (TPO-ab)-mediated activation of the
complement cascade. One hundred and forty-eight thyroid
autoantibody-positive women have been followed during their postpartum year. Seventy-five women remained euthyroid during this time whilst the remaining 73 showed one or more episodes of thyroid dysfunction. Fourteen women showed
hyperthyroid PPT, 23 showed a biphasic PPT and the remaining 36 showed hypothyroid PPT.
Hyperthyroid PPT was always transient but 29 of the 59 women with
hypothyroidism remained hypothyroid or still required
thyroxine replacement
therapy at the conclusion of the study. Thyroid
autoantibodies were measured by
enzyme-linked
immunosorbent assay (ELISA), free
triiodothyronine and free
thyroxine by the Amerlex M methods and
thyrotrophin by the Amerlite TSH (monoclonal) assay.
Complement component C3b, immobilized as a result of classical complement pathway activation in the presence of TPO/TPO-ab complexes in vitro, was measured by ELISA. Bioactive TPO-ab were calculated as the product of the C3 index and TPO-ab level. Basal levels of
complement C3 activation were seen in the euthyroid TPO-ab-positive women (C3 index 0.06 at delivery rising to 0.36 at 12 months postpartum; bioactive TPO-ab activity 0.4 kIU/l at delivery rising to 10.4 kIU/l at 12 months' postpartum (N = 75). These parameters were elevated progressively as the severity of the clinical syndrome increased. In 14
hyperthyroid PPT women the C3 index was 0.47 at 8 months' postpartum (bioactive TPO-ab activity = 20 kIU/I; p vs euthyroid group, NS).(ABSTRACT TRUNCATED AT 250 WORDS)