Purification and N-terminal sequence of a novel interferon-induced 100 kD protein secreted by mouse Ehrlich ascites tumor cells.

Abstract

Mouse Ehrlich ascites tumor cells secrete a 100 kD protein when they are stimulated by murine interferon-beta (2000 IU/ml). This 100 kD protein was purified from conditioned medium by chromatography on phenyl-Sepharose CL-4B, phosphocellulose, DEAE-Sephacel, and Vydac C4 columns and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A single species of 100 kD protein was isolated, and its N-terminal sequence analysis suggested that mouse Ehrlich ascites tumor cell-derived interferon-induced 100 kD protein is a novel protein.

• Authors: S Tominaga, J Utsumi
• Journal: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research (J Interferon Cytokine Res) Vol. 15 Issue 5 Pg. 417-20 (May 1995) ISSN: 1079-9907 [Print] United States
• PMID: 7648443 (Publication Type: Journal Article)

Chemical References

• Culture Media, Conditioned
• Neoplasm Proteins
• Recombinant Proteins
• Interferon-beta

Topics

• Amino Acid Sequence
• Animals
• Carcinoma, Ehrlich Tumor (metabolism)
• Chromatography, High Pressure Liquid
• Culture Media, Conditioned
• Electrophoresis, Polyacrylamide Gel
• Interferon-beta (pharmacology)
• Mice
• Molecular Sequence Data
• Neoplasm Proteins (isolation & purification, metabolism)
• Recombinant Proteins (pharmacology)
• Tumor Cells, Cultured