Abstract | PURPOSE: Previous studies from our laboratory have suggested that pooling of ammonium in tumor tissues may be caused by its inefficient removal due to the poor vasculature commonly found in tumors. The purpose of these experiments was to validate the relationship between tumor ammonium ion concentration and tumor blood flow, and to determine whether large concentrations of ammonium ion detected by Nuclear Magnetic Resonance (NMR) spectroscopy are either produced within the tumor or simply imported into the tumor through the blood stream. METHODS AND MATERIALS: To test this hypothesis, we reduced blood flow in subcutaneously grown Radiation Induced Fibrosarcoma-1 (RIF-1) tumors, either by creating partial ischemia with a bolus injection of hydralazine or by occlusion with surgical sutures. 14N and 31P NMR spectroscopy were used to detect the presence of ammonium, and to assess the bioenergetic status of the tumors, respectively. RESULTS: CONCLUSION: Our results support the hypothesis that a reduction in tumor blood flow is responsible for the accumulation of ammonium in tumors, and that detected ammonium originated from within the tumor.
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Authors | I Constantinidis, M P Gamcsik |
Journal | International journal of radiation oncology, biology, physics
(Int J Radiat Oncol Biol Phys)
Vol. 33
Issue 1
Pg. 143-9
(Aug 30 1995)
ISSN: 0360-3016 [Print] United States |
PMID | 7642412
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Fibrosarcoma
(blood supply, metabolism)
- Hydralazine
(pharmacology)
- Ligation
- Magnetic Resonance Spectroscopy
- Mice
- Mice, Inbred C3H
- Regional Blood Flow
(drug effects)
- Sarcoma, Experimental
(blood supply, metabolism)
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